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Pré-Publication, Document De Travail Année : 2020

A single master regulator controls asexual cell cycle division patterns in Toxoplasma gondii

Résumé

All apicomplexan parasites have complex life cycles exhibiting division characterized by a tightly regulated cell cycle control, resulting in the emergence of daughter parasites in possession of a single nucleus and a complete set of organelles. Apicomplexa have evolved efficient and distinctive strategies for intracellular replication where the timing of emergence of the daughter cells, a process termed “budding”, is a decisive element. However, the molecular mechanisms that provide the proper timing of parasite budding remain unknown. Using Toxoplasma gondii as a model Apicomplexa, we identified a master regulator that controls the timing of the budding process. We show that an ApiAP2 transcription factor, TgAP2IX-5, controls cell cycle events downstream of centrosome duplication including organelle division and segregation. TgAP2IX-5 binds to the promoter of hundreds of genes and controls the activation of the budding-specific cell cycle expression program. We show that TgAP2IX-5 regulates the expression of specific transcription factors that are necessary for the completion of the budding cycle. TgAP2IX-5 acts as a licensing factor that ensures that asexual proliferation continues by promoting the inhibition of the differentiation pathway at each round of the cell cycle. Therefore, TgAP2IX-5 is a master regulator that controls both cell cycle and developmental pathways.
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Dates et versions

hal-03107529 , version 1 (24-11-2020)
hal-03107529 , version 2 (12-01-2021)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

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Asma S. Khelifa, Cecilia Sanchez Guillen, Kevin M. Lesage, Ludovic Huot, Pierre Péricard, et al.. A single master regulator controls asexual cell cycle division patterns in Toxoplasma gondii. 2020. ⟨hal-03107529v1⟩
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