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Article Dans Une Revue Optics Letters Année : 2000

Membrane imaging by simultaneous second-harmonic generation and two-photon microscopy

Résumé

We demonstrate that simultaneous second-harmonic generation (SHG) and two-photon-excited fluorescence (TPEF) can be used to rapidly image biological membranes labeled with a styryl dye. The SHG power is made compatible with the TPEF power by use of near-resonance excitation, in accord with a model based on the theory of phased-array antennas, which shows that the SHG radiation is highly structured. Because of its sensitivity to local asymmetry, SHG microscopy promises to be a powerful tool for the study of membrane dynamics.
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Dates et versions

hal-02168812 , version 1 (10-11-2019)

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Laurent Moreaux, Olivier Sandre, Mireille Blanchard-Desce, Jerome Mertz. Membrane imaging by simultaneous second-harmonic generation and two-photon microscopy. Optics Letters, 2000, 25 (5), pp.320-322. ⟨10.1364/OL.25.000320⟩. ⟨hal-02168812⟩
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