The Mouse B- raf Gene Encodes Multiple Protein Isoforms with Tissue-specific Expression - Institut Curie Accéder directement au contenu
Article Dans Une Revue Journal of Biological Chemistry Année : 1995

The Mouse B- raf Gene Encodes Multiple Protein Isoforms with Tissue-specific Expression

Résumé

The c-Rmil/B-raf proto-oncogene is a member of the mil/raf family encoding serine/threonine protein kinases shown to be involved in signal transduction from the membrane to the nucleus. We isolated from a mouse brain library B-raf cDNAs containing a previously unidentified 36-base pair alternatively spliced exon located between exons 8 and 9 and, therefore, designated exon 8b. Human and mouse B-raf mRNAs also contain the 120-base pair alternatively spliced exon 10 previously described in the avian c-Rmil gene. Independent splicing of these two exons, located between the conserved region 2 (CR2) and the catalytic domain (CR3) gives rise to mRNAs potentially encoding four distinct proteins. By using specific sera generated against different portions of B-Raf, we identified at least 10 protein isoforms in adult mouse tissues. Some isoforms, in the range of 69-72 kDa, are not recognized by antisera directed against peptides encoded by exons 1 and 2, indicating the existence of B-Raf proteins with two different NH2 extremities. The other isoforms, in the range of 79-99 kDa, contain the amino acids encoded by exons 1 and 2, by either or both of the alternatively spliced exons, and, possibly, by another of the unidentified exon. Analysis of B-raf mRNA expression by reverse transcriptase-polymerase chain reaction and immunocharacterization of B-Raf proteins in different tissues of the adult mouse showed a tissue-specific pattern of B-Raf isoforms expression. Interestingly, isoforms containing amino acids encoded by exon 10 are specifically expressed in neural tissues. Taken together, these results suggest that distinct B-Raf proteins could be involved, in a tissue-specific manner, in signal transduction pathways.
Fichier principal
Vignette du fichier
PIIS0021925818899239.pdf (632.7 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-02525365 , version 1 (28-05-2021)

Licence

Paternité

Identifiants

Citer

Jean-Vianney Barnier, Catherine Papin, Georges Calothy, Alain Eychène, Odile Lecoq. The Mouse B- raf Gene Encodes Multiple Protein Isoforms with Tissue-specific Expression. Journal of Biological Chemistry, 1995, 270 (40), pp.23381-23389. ⟨10.1074/jbc.270.40.23381⟩. ⟨hal-02525365⟩
43 Consultations
51 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More