New case of c-src gene transduction: the generation of virus PR2257. - Institut Curie Accéder directement au contenu
Article Dans Une Revue Folia Biologica -Krakow- Année : 1994

New case of c-src gene transduction: the generation of virus PR2257.

J Briest'Anská
  • Fonction : Auteur
Josef Geryk
  • Fonction : Auteur
Ivan Karakoz
  • Fonction : Auteur
A Michailik
  • Fonction : Auteur
Jiří Nehyba
  • Fonction : Auteur
Bogdan A. Yatsula
  • Fonction : Auteur
Alla V. Rynditch
  • Fonction : Auteur

Résumé

PR2257 is a new replication-defective avian sarcoma virus which harbours in addition to the spliced version of the c-src gene also about 950 bp of no-coding cellular sequences located downstream from the c-src stop codon (Geryk et al., 1989). Comparison of the 950 bp region transduced by PR2257 with the chicken c-src cDNA (Dorai et al., 1991) and genomic sequences of the c-src 3' non-coding region from chicken and quail has shown that there are no additional introns. The c-src 3' non-coding region represents the largest c-src exon (No. 12) comprising about 2 kb. Absence of conserved open reading frames within this region in chicken and quail genomic DNAs excludes the possibility for coding a protein by these sequences. Also, the possibility was excluded that numerous endogenous virus-derived sequences identified in molecularly cloned PR2257 provirus played a role in the c-src transduction. After serial passaging of PR2257 virus in vivo a variant PR2257/16 was isolated. In PR2257/16, the size of the env gene was increased due to homologous recombination with a helper virus. In addition to mutations in the viral leader and the v-src coding region, a large deletion in transduced c-src 3' non-coding sequences was found in the PR2257/16 genome. The significance of genome modifications for selective advantage of this viral variant in vivo is discussed.
Fichier non déposé

Dates et versions

hal-02525381 , version 1 (30-03-2020)

Identifiants

  • HAL Id : hal-02525381 , version 1
  • PUBMED : 7895852

Citer

Philippe Dezélée, Jean-Vianney Barnier, J Briest'Anská, Josef Geryk, Ivan Karakoz, et al.. New case of c-src gene transduction: the generation of virus PR2257.. Folia Biologica -Krakow-, 1994, 40 (5), pp.211-23. ⟨hal-02525381⟩
18 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More