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Article Dans Une Revue Molecular crystals and liquid crystals science and technology. Section B, Nonlinear optics Année : 2000

Simultaneous second-harmonic generation and two-photon excited fluorescence microscopy

Résumé

We demonstrate that simultaneous second-harmonic generation (SHG) and two-photon excited fluorescence (TPEF) can be used to rapidly image membranes labeled with a lipophilic styryl dye. We have developed a model based on the theory of phased-array antennas which shows that the SHG radiation is highly structured and can be roughly of the same power as TPEF. This model provides a definition of a SHG cross-section which can be directly compared to the TPEF cross-section.
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Dates et versions

hal-02994185 , version 1 (07-11-2020)

Identifiants

  • HAL Id : hal-02994185 , version 1

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Laurent Moreaux, Olivier Sandre, Mireille Blanchard-Desce, Jerome Mertz. Simultaneous second-harmonic generation and two-photon excited fluorescence microscopy. Molecular crystals and liquid crystals science and technology. Section B, Nonlinear optics, 2000, 25 (1-4), pp.183-188. ⟨hal-02994185⟩
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