Simultaneous second-harmonic generation and two-photon excited fluorescence microscopy
Résumé
We demonstrate that simultaneous second-harmonic generation (SHG) and two-photon excited fluorescence (TPEF) can be used to rapidly image membranes labeled with a lipophilic styryl dye. We have developed a model based on the theory of phased-array antennas which shows that the SHG radiation is highly structured and can be roughly of the same power as TPEF. This model provides a definition of a SHG cross-section which can be directly compared to the TPEF cross-section.
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Moreaux et al MCLC S&T-SectionB Nonlinear optics 2000 Vol 25(1-4) 183-188 author ms.pdf (662.1 Ko)
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